| 1. | Screening of differentially expressed genes in placentas with hepatitis b virus infection by suppression subtractive hybridization technique
乙型肝炎病毒感染胎盘组织差异表达基因的筛选与确定 |
| 2. | Dominating methods in wheat gene cloning include homology - based cloning , map - based cloning , suppression subtractive hybridization , ddrt - pcr etc
目前，小麦基因的克隆方法主要有同源克隆、图位克隆、差减杂交、差异显示等。 |
| 3. | Among these techniques , the suppression subtractive hybridization ( ssh ) method has been shown to be quick and highly efficient , and it generates less false - positive clones
将实验方的双链cdna分成两组，分别与两种不同的接头连接( adaptor1andadaptor2r ) 。 |
| 4. | In virtue of the technique of suppression subtractive hybridization , we had successfully constructed a subtractive library of d . involucrata sprouting bract by taking homochronous leaf as driver in this study
本研究利用新近发展的抑制性差减杂交技术( ssh )成功构建了珙桐幼嫩苞片和叶片差减文库。 |
| 5. | In previous study , janghong have succeeded cloning 24 ests of mouse testis spermatogenic cell apoptosis - related gene by creating mouse cryptorchidism model and making use of suppression subtractive hybridization , and registered in genbank
在前期的研究工作中，本室姜宏等通过建立小鼠隐睾模型，运用抑制消减杂交法( suppressionsubtractivehybridization ， ssh )克隆出24个小鼠睾丸生精细胞凋亡相关基因的est ，并在genbank中登录。 |
| 6. | After the screening , the expression and identity of the gene for nadp - malic enzyme in leaves of aloe vera l . under salt stress had been done . methods : construction and screening of the subtractive library : to construct a cdna subtractive library of aloe vera l . under salt stress , the leaves of aloe vera l were removed from the seedlings which were treated with 300 mm nacl . suppression subtractive hybridization ( ssh ) was carried out and a subtractive library was constructed
盐胁迫下库拉索芦荟叶子中nadp -苹果酸酶基因的表达鉴定：为了检测芦荟盐胁迫下库拉索芦荟cdna消减又库的构建筛选及nadp一苹果酸酶基因的盐诱导表达中nadp一苹果酸酶基因‘刀叻心)的表达和nadp一苹果酸酶( nadp一ma1ieenzyme )蛋白的积累是否受着高盐的诱导，我们利用耐盐品种库拉索芦荟( a了口。 |
| 7. | Part 1 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) chapter 1 construction of subtractive cdna library of mitten crab ( eriocheir japonica sinensis ) ovary two subtracted cdna libraries of mitten crab ( eriocheir japonica sinensis ) ovaries at two successive developmental stages were constructed by suppression subtractive hybridization ( ssh )
第一部分中华绒螯蟹卵巢发育相关基因的筛选1中华绒螯蟹卵巢差减cdna文库的构建应用抑制性差减杂交技术，构建了中华绒螯蟹卵巢两个发育时期的差减cdna文库。 |
| 8. | A cdna subtractive library with high subtractive efficiency of repeated + gz exposures in rat brain was constructed with suppression subtractive hybridization ( ssh ) . the cdna subtractive library after amplification included 100 blue clones and 400 white clones , 75 ones of which were selected to prepare for plasmid . identification of the clones with restriction endonuclease cleavage showed most of them had been cloned to the vector
构建高消减效率的+ gz重复暴露大鼠脑cdna消减文库，扩增后cdna文库包含约400个白色克隆和100个蓝色克隆，克隆饱满清晰，随机挑取75个白色克隆，制备质粒后，以ecor酶切分析，表明大部分克隆入质粒载体。 |
| 9. | In this study , sd rats were used to establish the animal model of brain injury induced by repeated + gz exposure and suppression subtractive hybridization technique was adopted to screen the differentially expressed genes in rat brains of + gz exposure group . the aim of the study was to obtain preliminary experimental data for the molecular mechanisms of the brain injury
本研究利用大鼠重复+ 10gz暴露引发脑损伤的动物模型，观察脑的病理学改变；应用抑制性消减杂交技术筛选+ gz重复暴露大鼠脑的差异表达基因，旨在初步探讨+ gz重复暴露致脑损伤的分子机制。 |
| 10. | Furthermore , suppression subtractive hybridization ( ssh ) was employed for the isolation of cdna fragments for euonymus japonicus " zhuzi " differentially expressed genes , and forward suppression subtractive cdna library of cold - regulated genes was constructed . the seedlings of euonymus japonicus " zhuzi " treated with low temperature were as tester and untreated seedlings as driver . subtractive cdna library was differentially screened through cdna macroarray , six hundreds and four cdna clones were identified as cold specifically induced or highly expressed
( 5 )应用抑制差减杂交( suppressionsubtractivehybridization , ssh )方法，构建冷诱导表达的正向抑制差减cdna文库，低温处理的幼苗为tester ，常温处理为driver ，通过cdna微阵列差异筛选cdna文库，得到604个低温诱导或表达增强的候选克隆，对其中的84克隆进行dna测序，去除冗余的cdna ，在genbank中进行核酸和蛋白质同源性的比较和功能分析，共有36个单一序列，其中12个cdna在genbank数据库没有同源的序列。 |
